The facility currently averages about 3,500 sequence reads per week. Sanger CE read lengths are typically 700 bases, with a usual turnaround of two days. genomic clone ends (cosmids, BACs, PACs, phage).This instrument is usually reserved for SNP and microsatellite genotyping. For large scale products, a third ABI 3730XL canīe used. The Sanger Sequencing Facility provides high-quality capillary-based fluorescent sequencing on dual ABI 3730XL instruments. Oligo pick-up (and Sanger Sequencing sample drop off) locationsĬreate a TCAG (Sanger) sequencing account.Publications by Next-Generation Sequencing Facility.Next-Generation Sequencing Sample Submission Guidelines.Next-Generation Sequencing Facility Overview.Sanger Sequencing Sample Submission Guidelines.Automated library preparation on an Agilent Bravo automation system is available for some types of library preparation protocols. The facility also accepts sequencing-ready libraries under certain conditions. The Next-Generation Sequencing Facility offers consultation for project design (including involvement with the Informatics and Statistics Facilities), services for DNA or RNA library preparation for most species, and high-throughput sequencing using the Illumina NovaSeq 6000 and MiSeq platforms, as well as single molecule sequencing on the PacBio Sequel IIe and Oxford Nanopore PromethION platforms. The DNA Sequencing and Synthesis Facility offers high-quality capillary-based DNA sequencing, three next-generation sequencing technologies, and synthesis of conventional and labeled oligonucleotides to researchers in the academic, commercial and pharmaceutical communities.
TCAG Facilities Biobanking Cytogenomics and Genome Resources DNA Sequencing/Synthesis Genetic Analysis Informatics Microarray Analysis Statistical Analysis